荧光标记所依赖的化合物称为荧光物质。荧光物质是指具有共轭双键体系化学结构的化合物，受到紫外光或蓝紫光照射时，可激发成为激发态，当从激发态恢复基态时，发出荧光。荧光标记技术指利用荧光物质共价结合或物理吸附在所要研究分子的某个基团上，利用它的荧光特性来提供被研究对象的信息。荧光标记的无放射物污染，操作简便等优点，使得荧光标记物在许多研究领域的应用日趋广泛。人们利用利用荧光标记的多肽来检测目标蛋白的活性，并将其发展的高通量活性筛选方法应用于疾病治疗靶点蛋白的药物筛选和药物开发（例如，各种激酶、磷酸酶、肽酶等）。丹港生物经过长期开发，能够提供技术成熟的各种荧光标记多肽。
除了FRET荧光淬灭组合，丹港生物还可以提供包括FITC, FAM, TAMRA, Biotin等种类齐全的多肽荧光标记服务。下面是一些常见的多肽修饰荧光物质结构：
下表是一些荧光物质的激发光波长Ex(nm)和发射光波长Em(nm):
荧光基团
颜色
Ex(nm)
Em(nm)
Fluorescent dyes
颜色
Ex(nm)
Em(nm)
Alexa Fluor 350
Blue
346 nm
442 nm
TRITC (Rhodamine)
Yellow
550 nm
570 nm
Alexa Fluor 405
Blue
402 nm
421 nm
VIC
Yellow
538 nm
554 nm
Pacific Blue
Blue
410 nm
455 nm
NL557
Yellow
557 nm
574 nm
Dansyl
Blue
Cy3.5
Orange
581 nm
596 nm
Mca
Blue
Alexa Fluor 568
Orange
578 nm
603 nm
DAPI
Blue
340 nm
488 nm
ROX
Orange
575 nm
602 nm
AMCA
Blue
350 nm
450 nm
DyeMer 488/605
Orange
502 nm
609 nm
Cascade Blue
Blue
400 nm
420 nm
DyeMer 488/615
Orange
502 nm
615 nm
Fluoroblue
Blue
350 nm
450 nm
CAL Fluor(R) Red 590
Orange
569 nm
591 nm
Image-Blue
Blue
380 nm
465 nm
CAL Fluor(R) Red 610
Orange
569 nm
610 nm
Marina Blue
Blue
365 nm
460 nm
Cyanine 3.5
Orange
581 nm
596 nm
Pacific Blue
Blue
410 nm
455 nm
DyeMer 488/605
Orange
488 nm
605 nm
Cy2
Green
491 nm
509 nm
DyeMer 488/615
Orange
488 nm
615 nm
Alexa Fluor 430
Green
434 nm
539 nm
Lissamine Rhodamine
Orange
570 nm
590 nm
Alexa Fluor 488
Green
495 nm
519 nm
MFP555
Orange
560 nm
585 nm
Alexa Fluor 500
Green
503 nm
525 nm
Qdot 585
Orange
532 nm
585 nm
Alexa Fluor 514
Green
518 nm
540 nm
Qdot 605
Orange
<600 nm
605 nm
FITC
Green
490 nm
520 nm
ROX
Orange
575 nm
602 nm
Fluorescein
Green
494 nm
518 nm
Rhodamine Red-X
Orange
570 nm
590 nm
HNP
Green
290 nm
509 nm
Cy5
Red
646 nm
664 nm
5-FAM
Green
494 nm
522 nm
Cy5.5
Red
673 nm
692 nm
6-FAM
Green
492 nm
517 nm
CypHer5E
Red
644 nm
663 nm
BODIPY FL
Green
505 nm
513 nm
Alexa Fluor 594
Red
590 nm
617 nm
Cyanine 2
Green
492 nm
510 nm
Alexa Fluor 610
Red
612 nm
628 nm
DTAF
Green
495 nm
520 nm
Alexa Fluor 633
Red
632 nm
647 nm
DTAF/FITC
Green
495 nm
520 nm
Alexa Fluor 635
Red
633 nm
647 nm
dUTP-FITC
Green
495 nm
520 nm
Alexa Fluor 647
Red
650 nm
668 nm
FITC (Fluorescein)
Green
495 nm
520 nm
Alexa Fluor 660
Red
663 nm
690 nm
Hi FITC
Green
495 nm
520 nm
Texas Red
Red
596 nm
620 nm
JOE
Green
520 nm
548 nm
DyeMer 488/630
Red
502 nm
630 nm
MFP488
Green
501 nm
523 nm
Allophycocyanin
Red
650 nm
660 nm
Oregon Green 488
Green
496 nm
524 nm
PI
Red
535 nm
617 nm
Oregon Green 514
Green
511 nm
530 nm
Allophycocyanin (APC)
Red
650 nm
660 nm
Qdot 525
Green
488 nm
515 nm
Allophycocyanin-Cyanine 5
Red
633 nm
670 nm
TET
Green
521 nm
536 nm
Allophycocyanin-Cyanine 5.5
Red
633 nm
690 nm
CAL Fluor(R) Gold 540
Green
522 nm
544 nm
Atto 647
Red
645 nm
669 nm
NL493
Green
493 nm
514 nm
CAL Fluor(R) Red 635
Red
618 nm
637 nm
Cy3
Yellow
548 nm
562 nm
Cyanine 5
Red
650 nm
670 nm
Cy3B
Yellow
558 nm
572 nm
DyLight 647
Red
647 nm
673 nm
Alexa Fluor 532
Yellow
531 nm
554 nm
DyeMer 488/630
Red
488 nm
630 nm
Alexa Fluor 546
Yellow
556 nm
573 nm
Dyomics 647
Red
660 nm
673 nm
Alexa Fluor 555
Yellow
555 nm
565 nm
Lightcycler Red- 640
Red
625 nm
640 nm
HNP/Fast Red TR
Yellow
290 nm
562 nm
PBXL-1
Red
545 nm
666 nm
5-TAMRA
Yellow
546 nm
574 nm
PBXL-3
Red
614 nm
662 nm
Atto 550
Yellow
554 nm
576 nm
PerCP (Peridinin Chlorophyll)
Red
490 nm
675 nm
Cyanine 3
Yellow
550 nm
570 nm
PerCP-Cy5.5
Red
488 nm
695 nm
DyLight 547
Yellow
547 nm
574 nm
Qdot 655
Red
<620 nm
655 nm
HEX
Yellow
535 nm
556 nm
Quantum Red
Red
488 nm
660 nm
NED
Yellow
546 nm
575 nm
Quasar(R) 670
Red
647 nm
667 nm
Quasar(R) 570
Yellow
548 nm
566 nm
R-Phycocyanin (R-PC)
Red
614 nm
647 nm
