Antibody preparation technology service
DGpeptides Co.,Ltd provide peptide design and synthesis, polyclonal antibody preparation, monoclonal antibody preparation, antibody purification and antibody labeling services.
Peptide→ polyclonal antibody preparation
1. peptide synthesis:>85%(Usually >90%), 10 mg, 12-22AA
2. peptide-KLH (2-3 mg)
3. Deivery time:12-15weeks, Including two rabbits, four times immunizations
4. Antiserum purified by affinity chromatography
5. Goods:2-3 mg peptide;1-2 ml preimmune serum;2-5 mg Purified antibody;ELISA report(>1:40,000)
Peptide→ polyclonal antibody preparation
1. peptide synthesis:>85%(Usually >90%), 10 mg, 12-22AA
2. peptide-KLH (2-3 mg)
3. Deivery time:12-15weeks, Including two rabbits, four times immunizations
4. Antiserum purified by affinity chromatography
5. Goods:2-3 mg peptide;1-2 ml preimmune serum;2-5 mg Purified antibody;ELISA report(>1:40,000)
Peptide→ polyclonal antibody preparation
1. peptide synthesis:>85%(Usually >90%), 10 mg, 12-22AA
2. peptide-KLH (2-3 mg)
3. Deivery time:12-15weeks, Including two rabbits, four times immunizations
4. Antiserum purified by affinity chromatography
5. Goods:2-3 mg peptide;1-2 ml preimmune serum;2-5 mg Purified antibody;ELISA report(>1:40,000)
Peptide→ polyclonal antibody preparation
1. peptide synthesis:>85%(Usually >90%), 10 mg, 12-22AA
2. peptide-KLH (2-3 mg)
3. Deivery time:12-15weeks, Including two rabbits, four times immunizations
4. Antiserum purified by affinity chromatography
5. Goods:2-3 mg peptide;1-2 ml preimmune serum;2-5 mg Purified antibody;ELISA report(>1:40,000)
Gene→ monoclonal antibody preparation
1. Gene amplification, vector construction, Escherichia coli system protein ex
2. Spleening cells from mice with the highest serum titer were fused with SP2/0 and hybridoma cells were screened by ELISA
3. Deivery time:22-26weeks
4. Obtained Hybridoma cell line which stable secretion of specific antibodies after subclone,Subtype identification, Preparation of mouse ascites and purification of antibody Protein G
5. Goods:Plasmid;1 mg protein;50 µl preimmune serum;two hybridoma cell lines;2-5 mg monoclonal antibody(2 strains);ELISA(>1:80,000);Subtype identification report
Gene→ monoclonal antibody preparation
1. Gene amplification, vector construction, Escherichia coli system protein ex
2. Spleening cells from mice with the highest serum titer were fused with SP2/0 and hybridoma cells were screened by ELISA
3. Deivery time:22-26weeks
4. Obtained Hybridoma cell line which stable secretion of specific antibodies after subclone,Subtype identification, Preparation of mouse ascites and purification of antibody Protein G
5. Goods:Plasmid;1 mg protein;50 µl preimmune serum;two hybridoma cell lines;2-5 mg monoclonal antibody(2 strains);ELISA(>1:80,000);Subtype identification report
Phosphorylated peptide→monoclonal antibody preparation
1. peptide synthesis
1. phosphorylated peptide:>85%,10 mg,12-22AA
2. Non-phosphorylated peptide:>85%,10 mg,12-22AA
2. phosphorylated peptide-KLH or phosphorylated peptide-BSA(2-3 mg),Non-phosphorylated peptide-BSA;immunization, antiserum collection, ELISA test, Cell fusion and hybridization, subtype identification, subcloning and cell cryopreservation, Preparation of mouse ascites and purification of antibody Protein G(2 strains)
3. Deivery time:21-26weeks
4. Goods:50 µl preimmune serum;2-3 mg peptide(Non-modified and phosphorylated peptides);2-5 mg Phosphorylated antibody(2 strains);ELISA test(>1:80,000);Subtype identification report
* Extra service
· 4 weeks:One additional immunization and two blood collection sessions are provided if you need 80-100ml polyclonal antibody or monoclonal antibody serum
· Affinity chromatography purification:25 ml affinity purification of serum,Includes affinity chromatographic columns for purification
· ELISA test: ELISA tests are performed on specified blood samples or purified antibodies if needed
· Western test:Specific detection of antiserum or purified antibodies are performed if needed
· Immunohisto chemistry:The specific antigen recognition of purified antibody in pathological section was detected if needed
