Amide Cyclization is other important way to form peptide cyclization and helix. Depending on its functional groups, a peptide can be cyclized in four different ways: head-to-tail (C-terminus to N-terminus), head-to-side chain, side-chain-to-side-chain, or side chain-to-tail.

Head-to-Tail Amide Cyclization

In order to form head-to-tail aminde cyclization, we use 2cl-resin. Protect the a-amino group using TFA, DDE group etc. and make the cleavage using low concentration TFA, we can manufacture high quality head-to-tail aminde cyclization peptide with high success rate.

Side-Chain-to-Side-Chain Amide Cyclization

Side-chain-to-side-chain aminde cyclization requires that there are more than two Lysine or Ornithine in the sequence. TGpeptide can make the side-chain-to-side-chain aminde cyclization before cleavage and after cleavage.

Side Chain-to-Tail Amide Cyclization

Side chain-to-tail amide cyclization can be formed between side chain amide from Lys or Orn and C-terminal carboxyl group if these amino acids exist in peptide sequence. Using 2-CTC-resin, protecting the side chain amino group using TFA, DDE group etc. and making the cleavage using low concentration TFA, we can manufacture high quality Side chain-to-tail amide cyclization peptide with high success rate.

Side Chain-to-Head Amide Cyclization

Side chain-to-head amide cyclization can be formed between side chain carboxyl group from Asp or Glu and N-terminal amide group if these amino acids exist in peptide sequence. Using 2-CTC-resin, protecting the side chain carboxyl group using odmab and de-protect odmab specifically, then making the cleavage using low concentration TFA, we can manufacture high quality Side chain-to-head amide cyclization peptide with high success rate.